- A 150 cm3 conical flask was taken.
- 10.00 ± 0.01 cm3 of the aloe vera juice was transferred to it using a graduated pipette.
- The flask was set on the water bath and the temperature was adjusted to 35.00 C.
- The aloe vera juice was then heated using the water bath for 10 minutes.
- After 10 minutes, the flask was removed from the water bath.
- 2.00 cm3 of DPPH solution was added to it using a 10.00 cm3 graduated pipette.
- 2.00 cm3 of ethanol was added to the same.
- The colorimeter was calibrated at 517 nm using ethanol as the blank solution.
- The absorbance of the solution in the flask using a cuvette at 517 nm.
- The solution in the flask was kept for 1 hour to allow the antioxidants in the juice to react with DPPH.
- After 1 hour, the absorbance of the solution was recorded again at 517 nm.
The same process was repeated for other temperatures – 30.00 C (room temperature; water bath was not used), 40.00 C, 45.00 C, 50.00 C and 55.00 C. At each temperature, the data was collected in seven trials. Raw data: