Kitchen chemistry and cooking science is one of the most wide and major application of Chemistry. All the food products that we use starting from different spices to food additives are organic compounds and thus cooking is basically an organic reaction they go through. For example, change of color of green vegetables while cooking, souring of milk, caramelization of sugar are all examples of organic reactions. Thus, changes in chemical composition of substances during cooking has always been a major topic of interest in Chemistry. Due to the global pandemic, when access to school laboratory was not there, selecting and identifying a appropriate topic for the Chemistry Internal Assessment became an issue especially when I was determined to opt for an IA that uses experimental data. So, I chose to base my investigation on chemicals that are used in our daily life. Focusing on the fact that compounds change their molecular structure as well as composition during cooking, I decided to understand how it happens and what factors may impact it. I chose to deal with vinegar as that is one of the most widely used ingredients for cooking. Heating is an essential condition of cooking. Almost all foods that we prepare involves thermal exposure of chemicals in some or the other way. So, I decided to explore how exposure to temperature would alter the chemical composition of vinegar and to what extent.
How does the molar concentration of ethanoic acid (CH3COOH) in mol dm-3 within vinegar depends on the temperature to which it is heated temperature, determined using acid base titration with a primary standard NaOH solution?
Vinegar is a commercially used aqueous solution of ethanoic acid. As per FDA (Food and Drug Adminstration), vinegar must contain ethanoic acid within the range of 4.00% to 8.00% (volume by volume). It means that 100 cm3 of vinegar should contain 4.00 cm3 to 8.00 cm3 of ethanoic acid. The most widely used vinegar is Apple Cider Vinegar which is named based on the fact that it is made from apple. Vinegar is used as a souring agent and is used in marination of meats as it aids in the breaking down of polypeptide chains into smaller uses and thus makes it easier for the meat to become tender. Researches reports that vinegar has various medical benefits as well. Journals of medical researches reveals that direct consumption of vinegar may be beneficial to treat health conditions like hyperacidity, Type-II Diabetes and so on.
Vinegar is prepared in a biological process. The biochemical pathway consists of preparing vinegar involves two enzymatic pathways:
C6H12O6 (glucose) ----→ 2 C2H5OH (aq) +2 CO2 (g)
Glucose undergoes fermentation to form ethanol and release carbon dioxide. This step is a breakdown process where a large organic molecule of glucose decarboxylates (loses CO2) to produce ethanol.
2 C2H5OH (aq) + O2 (g) ---→ 2 CH3COOH
Here ethanol when exposed to oxygen in air oxidizes to ethanoic acid. The oxidation number of C increases from -2 in ethanol to 0.
The first step is anaerobic as it happens in absence of oxygen while the second step needs presence of oxygen. Both the steps involve the use of enzymes secreted from the microbes present within the fruit which undergoes fermentation. As both of them are enzyme catalyzed reactions, they occur at an optimum temperature and also requires a definite pH level. The ethanoic acid made is diluted and a dilute solution of vinegar containing ethanoic acid at 4% to 8% by volume is then used as vinegar.
Ethanoic acid is a weak organic acid and reacts with the strong base NaOH to produce the salt- Sodium ethanoate and water. This is a neutralization reaction and can thus be used as a basis of the analytical process to deduce the concentration of ethanoic acid from an acid base titration.
CH3COOH (aq) + NaOH (aq) -------→ CH3COONa (aq) + H2O ...........(equation - 1)
The salt formed is a basic salt as ethanoic acid is a weak acid and NaOH is a strong base. Thus, the pH at equivalence point will lie above 7.00 and thus phenolphthalein can be used as a suitable indicator for this titration. Here, the analyte is ethanoic acid and the titrant is NaOH. Thus, the color changes from colorless (in acidic medium) to pink (in basic medium).
The vinegar solutions will be heated to various temperatures using a water bath. After being heated, the solutions were titrated with a solution of NaOH of known concentration. The burette reading will be used to calculate the number of moles of NaOH consumed and thus the moles of ethanoic acid present. This will then be used to determine the concentration of ethanoic acid. Following this, a scatter plot will be used to elucidate the correlation of temperature and concentration of ethanoic acid in vinegar.
The main purpose of the investigation is to determine the molar concentration of ethanoic acid in vinegar. It can be done using a pH curve instead of an acid base titration. That would give us more accurate result. Moreover, concentration of ethanoic acid can also be determined spectrophotometrically by measuring the absorbance of the molecule at a wavelength at which it displays maximum absorbance. Since ethanoic acid is a colorless liquid, it would show absorbance in the ultra violet region and thus a UV-Visible spectrophotometer is required for this. However, the first method was discarded as a pH probe was not accessible and the lack of a UV-Visible spectrophotometer did not allow to use the second method.
In a medical study on the anti-bacterial and anti-glycemic effect of Vinegar, it was found that the increase of temperature reduces the concentration of ethanoic acid in vinegar. The study is titled as – “Vinegar: Medicinal Uses and Antiglycemic Effect” by Carol S. Johnston and Cindy A.Gaas published in the journal – “Medscape General Medicine”.
The temperature at which vinegar is heated has no correlation with the molar concentration of ethanoic acid in the vinegar.
As temperature increases, the molar concentration of ethanoic acid increases. With heating, the volume of water evaporates and thus the solution becomes more concentrated. Thus, molar concentration of ethanoic acid in vinegar would increase.
Temperature of Vinegar: A water bath was set to the required temperature and then the samples were incubated inside it. A thermometer was used for each sample to ensure the required temperature is reached. The cooking temperature usually differs from a range of 40°C to 100°C. So, the temperature measured were 20°C, 40°C, 60°C, 80°C and 100°C.
Concentration of Ethanoic Acid (CH3COOH) in Vinegar: The vinegar samples were titrated against NaOH solution of known concentration. The change in the burette readings were recorded. Using the volume of NaOH required as obtained from the burette reading, the moles and the stoichiometric ratio in which ethanoic acid reacts with NaOH, the molar concentration of ethanoic acid was deduced. The molar concentration was measured in the unit – moldm-3.
150 cm3 aqueous solution of NaOH with the same concentration was prepared and stored. The solution was stirred regularly and whenever needed.
150.00 cm3
150.00 cm3
1000.00 cm3
15.00 cm3
Burette (50cm3)
0.10 cm3
± 0.05 cm3
Graduated pipette – 10.00 cm3
0.10 cm3
± 0.05 cm3
Graduated Cylinder (50 cm3)
2.00 cm3
± 1.00 cm3
Graduated Cylinder (10 cm3)
0.20 cm3
± 0.10 cm3
Test tube (100 cm3)
Conical Flask (100 cm3)
10 cm3
± 5 cm3
Glass Beaker (250 cm3)
25 cm3
± 12.5 cm3
Ethical Issues:
Preparation of 100 cm3 of 1.00 mol dm-3 NaOH solution
Number of Moles = concentration × Volume = 1.00 × \(\frac{100}{1000}\) = 1.00 × 0.10 = 0.10 moles Mass of solid NaOH required = moles mass = 0.10 × (23.00 + 16.00 + 1.01 + 1.01) = 4.00g
For Temperature: 20.00°C:
For Trial - 1, Difference in burette reading
= Final burette reading – Initial burette reading
= 4.20 ± 0.05 cm3 – 0.00 ± 0.05 cm3 = 4.20 ± (0.05 + 0.05) cm3 = 4.20 ± 0.10 cm3
Mean burette reading = \(\frac{Trial-1+Trial-2+Trial-3+Trial-4+Trial-5}{5}\)
= \(\frac{4.20(±0.05)+4.00(±0.05)+4.60(±0.05)+4.70(±0.05)+4.20(±0.05)}{5}\) = 4.34 ± 0.05 cm3
As no concordant reading has been obtained, the arithmetic mean has been considered instead of the precise reading.
Mean difference in Burette Reading (± 0.10cm3)
Molar concentration of Acetic Acid ( × 10-2 mol dm-3)
For temperature 20.00 ± 0.50°C
Mean burette reading = 4.34 ± 0.10 cm3
Volume of NaOH consumed = mean burette reading = 4.34 ± 0.10 cm3
Moles of NaOH used = \(\frac{molar\ concentration\ of\ NaOH\ solution\ used\ in\ mol\ dm^{-3}×Volume\ of\ NaOH\ concumed\ in\ cm^3}{1000}\)
\(=\frac{1.00×4.34}{1000}\) = 0.0043 moles
Moles of ethanoic acid = moles of NaOH consumed (as they react in the mole ratio 1:1) = 0.0043 moles
Molar concentration of ethanoic acid = \(\frac{moles\ of\ ethanoic\ acid}{total\ volume\ of\ the\ solution\ in\ dm^3}\)
\(=\frac{0.0043}{\frac{5}{1000}}\) = 0.8680 = 86.80 × 10-2 mol dm-3
Mass of NaOH taken = 4.00 ± 0.01 g
Fractional error in concentration \((\frac{Δc}{c})=\frac{Δn}{n}+\frac{ΔV}{V}=\frac{±0.01}{4.00}+\frac{±0.10}{100.00}\) = ± 0.0035
Fractional error in moles of ethanoic acid
\(\frac{Δc_{ethanoic\ acid}}{c_{ethanoic\ acid}}=\frac{Δn}{n}+\frac{Δvolume\ of\ vineger}{Volume\ of\ vinegar}\) = ± 0.0265 + \(\frac{±0.05}{5.00}\) = ± 0.0365
Percentage error in concentration of ethanoic acid = ± 0.0365 × 100 = ± 3.65
The bottle of Apple Cider Vinegar used claims to have 5.00 % ethanoic acid as mentioned in the label. This means that 100.00 cm3 of this vinegar has 5.00 cm3 of ethanoic acid.
Mass of ethanoic acid = Volume × Density = 5.00 cm3 × 0.997 g cm-3 = 4.985 g
Molar concentration = \(\frac{\frac{mass}{molar\ mass}}{\frac{volume}{1000}}\) = \(\frac{\frac{4.985}{48.00}}{\frac{100}{1000}}\) × 100 = \(\frac{1.04-0.8680}{1.04}\) × 100 = 16.53
There is a percentage error 16.53 % which also indicates that there is a systematic error in this investigation.
Decrease in Concentration of Acetic Acid (mol.dm-3 )
For increase of temperature 20.00 ± 0.50o C to 40.00 ± 0.50o C,
Decrease in Concentration of ethanoic acid (mol.dm-3 )
= concentration of ethanoic acid at 20.00 ± 0.50°C – concentration of ethanoic acid at 40.00°C
= 0.8680 – 0.7960 = 0.0720 mol dm-3
The graph above, shows the decrease in concentration of Acetic acid in vinegar as concentration is increases. Temperature is taken along the x axis as it is the independent variable and the decrease in concentration of Acetic Acid is taken in the y axis as it the dependent variable. The values of decrease in increase temperature are very inconsistent. Although, an average decrease in concentration was found to be 0.0770 moles. This average predicts that as a temperature of 20°C is increased, the concentration of Acetic Acid in vinegar will decrease by 0.0770 moles.
The trend shows that the molar concentration of ethanoic acid decreases with the increase in the heating temperature. Applying the understanding that with rise of temperature, the water would start evaporating at a faster rate and thus volume of water would decrease eventually increasing the concentration of ethanoic acid, this trend cannot be justified. Hence, it is clear that the reason behind the decrease is not an outcome of a physical process but is related to some chemical reaction that ethanoic acid may have undergone. Carboxylic acids on heating undergoes thermal decarboxylation to produce a hydrocarbon and carbon dioxide. Ethanoic acid is also a weak carboxylic acid and may undergo the same process.
CH3COOH ----→ CH4 + CO2 (g)
With the increase in temperature, the rate of decomposition increases and thus the amount of ethanoic acid in the mixture decreases. Thus, there is lesser number of moles of ethanoic acid in the vinegar solution titrated. This also explains why the volume of NaOH required for the neutralization of ethanoic acid in the mixture decreases as temperature is increased. Table-5 (raw data table) clearly shows a decrease in the value of mean burette reading and that clearly supports this fact.
How does the molar concentration of ethanoic acid (CH3COOH) in mol dm-3 within vinegar depends on the temperature to which it is heated temperature, determined using acid base titration with a primary standard NaOH solution?
The molar concentration of ethanoic acid (CH3COOH) in mol.dm-3 in vinegar is inversely proportional to the temperature. Furthermore, it is concluded that the rate of change of concentration is not uniform throughout the change in temperature. The maximum rate of change of concentration is obtained between 40°C to 60°C and the minimum rate of change of concentration is obtained between 60°C and 80°C.
To investigate further, I would like to study how temperature affects other bio chemical reactions. Souring of milk is a chemical reaction which leads to formation of lactic acid from the hydrolysis of lactose present within it. I would like to heat the milk to different temperatures and then add a definite volume of souring agent (lime water) to it. This will result in the production of lactic acid within it. The volume of lactic acid produced can be deduced using an acid base titration with NaOH solution of known concentration. Thus, we can determine the mass of lactic acid produced on addition of a souring agent as a function of temperature.